Conformational Characterization of α-Mycolic Acid in a Monolayer Film by the Langmuir−Blodgett Technique and Atomic Force Microscopy
| Autor: | Toyoko Imae, Takeshi Hasegawa, and Jujiro Nishijo, Katsuya Funayama§ and, Motoko Watanabe |
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| Rok vydání: | 2000 |
| Předmět: | |
| Zdroj: | Langmuir. 16:7325-7330 |
| ISSN: | 1520-5827 0743-7463 |
| Popis: | Conformational changes of α-mycolic acid (α-MAMAI, isolated from Mycobacterium avium−M. intracellulare complex) in its monolayer at different surface pressures were investigated by using the Langmuir−Blodgett (LB) technique and atomic force microscopy (AFM). Three representative monolayers that correspond to the gas, liquid-expanded, and solid states were prepared and transferred onto freshly cleaved mica. The monolayer LB films of α-MAMAI were examined by AFM in an angstrom resolution in the thickness direction. The observation of topography and cross section of the monolayers proposed a series of schematic pictures for the lateral arrangement of the alkyl chains of α-MAMAI in the monolayer at different surface pressures. The proposed molecular conformations were consistent with the surface pressure−area isotherm. The results suggested that the α-MAMAI molecules partially took a flat-on stance in the gas-state monolayer and that the full extension of the longer alkyl chain of the molecule in the monolaye... |
| Databáze: | OpenAIRE |
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