Development of Self-incompatible Lines with Improved Seed Quality in Winter Oilseed Rape (Brassica napus L.) for Hybrid Breeding

Autor:	R. Koprna, Vratislav Kučera, Miroslav Klíma, M. Vyvadilova, O. Kolovrat
Rok vydání:	2005
Předmět:	media_common.quotation_subject Brassica Plant Science Biology biology.organism_classification chemistry.chemical_compound Horticulture chemistry Inflorescence Erucic acid Genetic resources Glucosinolate Genetics Doubled haploidy Reproduction Hplc method media_common
Zdroj:	Czech Journal of Genetics and Plant Breeding. 41:105-111
ISSN:	1805-9325 1212-1975
DOI:	10.17221/3668-cjgpb
Popis:	Doubled haploid (DH) self-incompatible (SI) regenerants with improved seed quality were derived after two improvement crossing cycles with different “00” quality donors. The original genetic resource of recessive sporophytic SI was characterised by a high glucosinolate (GSL) and erucic acid content. SI regenerants of satisfactory seed quality parameters were obtained from the second cycle of crossing. Total number of derived SI plants was 87, i.e. 38.2% out of 228 obtained fertile doubled haploid regenerants developed in 2004. Nine plants out of the analysed 45 SI DH regenerants had the erucic acid content lower than 2%. Five out of these nine plants, which were analysed by HPLC method, had the glucosinolate content lower than the limit 18 µmol/g at 9% seed moisture. The method based on the spraying of inflorescences with 5% NaCl to enable reproduction of SI lines was verified.
Databáze:	OpenAIRE
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