Random and targeted transgene insertion in Caenorhabditis elegans using a modified Mos1 transposon
| Autor: | Christian Frøkjær-Jensen, Erik M. Jorgensen, Stephane Flibotte, Mihail Sarov, Matthew L. LaBella, Jon Taylor, M. Wayne Davis, Andrei Pozniakovsky, Donald G. Moerman |
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| Rok vydání: | 2014 |
| Předmět: |
Genetics
Caenorhabditis briggsae Transposable element 0303 health sciences Tn3 transposon genetic structures biology Cell Biology biology.organism_classification Sleeping Beauty transposon system Biochemistry P element 03 medical and health sciences 0302 clinical medicine Composite transposon Simple transposon Molecular Biology 030217 neurology & neurosurgery Transposase 030304 developmental biology Biotechnology |
| Zdroj: | Nature Methods. 11:529-534 |
| ISSN: | 1548-7105 1548-7091 |
| DOI: | 10.1038/nmeth.2889 |
| Popis: | We have generated a recombinant Mos1 transposon that can insert up to 45-kb transgenes into the Caenorhabditis elegans genome. The minimal Mos1 transposon (miniMos) is 550 bp long and inserts DNA into the genome at high frequency (~60% of injected animals). Genetic and antibiotic markers can be used for selection, and the transposon is active in C. elegans isolates and Caenorhabditis briggsae. We used the miniMos transposon to generate six universal Mos1-mediated single-copy insertion (mosSCI) landing sites that allow targeted transgene insertion with a single targeting vector into permissive expression sites on all autosomes. We also generated two collections of strains: a set of bright fluorescent insertions that are useful as dominant, genetic balancers and a set of lacO insertions to track genome position. |
| Databáze: | OpenAIRE |
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