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Objective. To investigate the resuscitative efficacy of haemoglobin vesicles (HbVs) for severe postpartum haemorrhage (PPH) using pregnant rabbits. Design: Animal research study Setting: Animal laboratory, National Defense Medical College, Japan Population: Twenty-five female New Zealand white rabbits at late gestation Methods. Pregnant rabbits underwent caesarean section; uncontrolled haemorrhage was induced by transecting the uterine artery to establish a severe PPH model. During the first 30 min (or until the bleeding volume reached 100 mL), all rabbits received 6% hydroxyethyl starch (HES) infusion. Thereafter, rabbits received any of the three isovolemic fluid resuscitations every 5 min: red blood cells (RBCs) with platelet-poor plasma (RBC/PPP) (vol/vol=1:1, n=8), 6% HES (n=7), or HbVs with 25% human serum albumin (vol/vol=4:1, n=10). After 60 min (or when the bleeding volume reached 200 mL), we performed surgical haemostasis and monitored the rabbit survival for 12 hours. Main Outcome Measures: Survival time for severe postpartum haemorrhage Results. During the first 30 min, all rabbits showed severe anaemia (Hb |
