Effect of winter-flooding on methanogenic archaeal community structure in paddy field under organic farming
| Autor: | Susumu Asakawa, Dongyan Liu, Toyoaki Ito, Makoto Kimura, Kazunori Akita, Takeshi Watanabe, Chika Suekuni, Masanori Saito |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
chemistry.chemical_classification
biology Intensive farming business.industry fungi Community structure food and beverages Soil Science Plant Science biology.organism_classification chemistry Agronomy Agriculture Organic farming Environmental science Paddy field Organic matter business Temperature gradient gel electrophoresis Archaea |
| Zdroj: | Soil Science and Plant Nutrition. 58:553-561 |
| ISSN: | 1747-0765 0038-0768 |
| DOI: | 10.1080/00380768.2012.726598 |
| Popis: | Paddy field is a major emission source of methane. Methane is the terminal product of anaerobic decomposition of organic matter and generated by methanogenic archaea under flooded conditions in paddy fields. This study aimed to reveal the effect of winter flooding on methanogenic archaeal community structure in paddy fields of Andosols under organic farming. Soil samples were collected from experimental paddy fields in the Field Science Center, Tohoku University, for two years. They were under flooding conditions during winter with organic farming, under non-flooding conditions during winter with organic farming and under non-flooding conditions during winter with conventional farming (non-organic farming). Polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) analysis of methanogenic archaeal 16S rRNA gene revealed that the DGGE patterns were nearly the same irrespective of the treatment and sampling times. Twenty-three bands were observed from each treatment and 4, 13 and 6 sequen... |
| Databáze: | OpenAIRE |
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