Why is the ratio of H2/acetate over 2 in glucose fermentation by Caldicellulosiruptor saccharolyticus?
| Autor: | Fang Zhang, Yan-Shan Wang, Raymond J. Zeng, Nan Shen, Xiang-Ning Song |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Ethanol
biology Renewable Energy Sustainability and the Environment Chemistry Energy Engineering and Power Technology Biomass Optical density Condensed Matter Physics biology.organism_classification chemistry.chemical_compound Fuel Technology Biochemistry Fermentation High ratio Caldicellulosiruptor saccharolyticus Nuclear chemistry |
| Zdroj: | International Journal of Hydrogen Energy. 38:11241-11247 |
| ISSN: | 0360-3199 |
| DOI: | 10.1016/j.ijhydene.2013.06.091 |
| Popis: | The ratio of H 2 /acetate in glucose fermentation shall be equal or less than 2. However, the ratio over 2 is found in the literature. Two possible reasons were proposed in this study: acetate oxidation or biomass growth via acetyl-CoA. In order to find out the right reason, glucose fermentation by Caldicellulosiruptor saccharolyticus was investigated. Under different glucose concentrations (1.0 g/L, 2.0 g/L, 3.5 g/L and 7.5 g/L), the Optical Density (OD) reached a maximum value of 0.35, 0.48, 0.53 and 0.55, respectively. It was found that the ratios of H 2 /acetate under different glucose concentrations were all greater than 2. When CH 3 - 13 COOH was added to the system, 60% of CH 3 - 13 COOH was converted to isotope ethanol. About the same amount of 13 CO 2 (0.01 mmol) was detected in both the control and isotopic experiments, illustrating acetate oxidation didn't occur in this study. The corrected ratio of H 2 /acetate after the compensation from biomass growth was around 2, demonstrating the biomass growth from acetyl-CoA was the right reason for the abnormal high ratio. |
| Databáze: | OpenAIRE |
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