Molecular Recognition Analyzed by Observing Intramolecular Interconversion with EPR Spectroscopy

Autor:	Paul Schuler, Martin Jäger, Hartmut B. Stegmann, Antal Rockenbauer
Rok vydání:	1998
Předmět:	Chemistry Intermolecular force Analytical chemistry Protonation General Chemistry Site-directed spin labeling Nuclear magnetic resonance spectroscopy law.invention Crystallography Molecular dynamics Molecular recognition law Intramolecular force Electron paramagnetic resonance
Zdroj:	Zeitschrift für Naturforschung B. 53:1511-1519
ISSN:	1865-7117 0932-0776
DOI:	10.1515/znb-1998-1215
Popis:	Molecular recognition between Rebek’s cleft R and 4-(2-benzimidazolyl)-aminomethyl-2,6-di-rm-butyl-phenol (Bi) is investigated by means of EPR spectroscopy. Their specific inter-molecular interactions are distinguished from non-specific interactions represented by mere protonation on addition of benzoic acid. Both interactions are related to intramolecular in­ terconversions at the C∞-atom of Bi*, where a two-jump mechanism is found for free and protonated Bi* and a two-site exchange for the complexed ligand. While NMR spectroscopy indicates the existence of 1:1 and 1:2 receptor-substrate complexes, the faster time scale of EPR spectroscopy allows to identify one 1 : 2 as well as three 1 : 1 associates and to elucidate their formation conditions. Based upon the corresponding hf parameters, binding modes and sites are proposed for the key-in-the-lock system.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::bd2d0b593d226d5398a27bf20a698e2e https://doi.org/10.1515/znb-1998-1215 Zobrazit plný text záznamu