Effects of Toxic Organotin Compounds on Bacteria Investigated by Micro‐Raman Spectroscopy
| Autor: | Ganesh D. Sockalingum, Pascal Picart, Ph. Daniel, Gérald Thouand, Marie-José Durand, F. Ergan, Th. Charrier, Loubna Bendriaa, Isabelle Adt, Michel Manfait |
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| Rok vydání: | 2008 |
| Předmět: |
Pollutant
biology Chemistry biology.organism_classification medicine.disease_cause Atomic and Molecular Physics and Optics Toxic chemical Analytical Chemistry Micro raman spectroscopy symbols.namesake Environmental chemistry Tributyltin chloride symbols medicine Raman spectroscopy Spectroscopy Escherichia coli Bacteria Nuclear chemistry |
| Zdroj: | Spectroscopy Letters. 41:19-28 |
| ISSN: | 1532-2289 0038-7010 |
| DOI: | 10.1080/00387010701799621 |
| Popis: | Within the framework of the detection of toxic substances using specific bacteria, the effect of the toxic chemical compound tributyltin chloride (TBT) on the bacterium Escherichia coli TBT3 was investigated using a noninvasive approach based on micro‐Raman spectroscopy. Various cellular densities with optical density (OD) ranging from 1 to 4 and harvested at different growth phases were induced for 1 h with different TBT concentrations: 1, 3, 5, and 10 µM. As an original contribution, the Raman results show that, in these conditions, the vibrational signatures of bacteria were affected by the presence of the toxic TBT compound, mainly in the signal characteristics from carbohydrates and lipids. Hierarchical cluster analysis (HCA) carried out on the Raman data in these regions makes it possible to classify the spectra in three groups according to the TBT concentration, confirming the progressive influence of the chemical pollutant on the bacterium. This work shows the potentiality for the Raman t... |
| Databáze: | OpenAIRE |
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