Hepatic stellate cell line modulates lipogenic transcription factors
| Autor: | Fátima Theresinha Costa Rodrigues Guma, Elena Aida Bernard, Mariana Ferreira da Silva Franceschi, Eduardo Linck Machado Guimarães, Radovan Borojevic, Regina Maria Vieira da Costa Guaragna, Rogério Margis, Claudia Marlise Balbinotti Andrade |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
chemistry.chemical_classification
medicine.medical_specialty Hepatology Liver cytology Peroxisome proliferator-activated receptor Biology Cell biology chemistry.chemical_compound Endocrinology chemistry Cell culture Internal medicine Enhancer binding Adipocyte Hepatic stellate cell medicine Myofibroblast Transcription factor |
| Zdroj: | Liver International. 27:1255-1264 |
| ISSN: | 1478-3223 |
| DOI: | 10.1111/j.1478-3231.2007.01578.x |
| Popis: | Background/Aims: Pre-adipocyte differentiation into adipocyte is a terminal differentiation process triggered by a cascade of transcription factors. Conversely, hepatic stellate cells (HSC) can switch between lipid storing and the myofibroblast phenotype in association with liver fibrotic processes. Here, adipogenic/lipogenic-related transcription factors and downstream-regulated genes were evaluated in a murine HSC cell line. GRX-HSC cells are transitional myofibroblasts that differentiate into lipocytes following retinol or indomethacin treatment. Methods/Results: Specific mRNAs were quantified by a real-time polymerase chain reaction after 24 h or 7 days of cell culture with indomethacin or retinol. Proliferator-activated receptorγ and Pex16 transcripts were increased either by retinol or indomethacin. Retinol induced a minor increase in C/enhancer binding proteinα transcripts, while only indomethacin increased adipsin transcripts. Conclusions: Our results showed that the myofibroblast to lipocyte phenotype switch follows partially different transcriptional pathways, according to the effector. Retinol induces lipid synthesis and storage without affecting characteristic adipocytic genes, while indomethacin treatment restores the lipocytic phenotype with increased adipisin expression. |
| Databáze: | OpenAIRE |
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