Highly Efficient Visible‐Light‐Induced Photocleavage of DNA by a Ruthenium‐Substituted Fluorinated Porphyrin

Autor: Shawn Swavey, Anna McCrate, Michelle Cunningham, Mark G. Nielsen
Rok vydání: 2009
Předmět:
Zdroj: European Journal of Inorganic Chemistry. 2009:1521-1525
ISSN: 1099-0682
1434-1948
DOI: 10.1002/ejic.200900080
Popis: A new porphyrin, meso-5-(pentafluorophenyl)-10,15,20-tris(4-pyridyl)porphyrin, has been synthesized. Coordination of two [Ru(bipy)2Cl]+ moieties (where bipy = 2,2′-bipyridine) to the pyridyl nitrogen atoms in the 10,15-positions gives the target complex. Electronic transitions associated with the ruthenium–porphyrin include an intense Soret band and four less intense Q-bands in the visible region of the spectrum. An intense π–π* transition in the UV region associated with the bipyridyl groups and a metal-to-ligand charge-transfer (MLCT) band appearing as a shoulder to the Soret band are also observed. Electrochemical properties associated with the complex include a redox couple in the cathodic region with E1/2 = –0.84 V vs. Ag/AgCl attributed to the porphyrin and a redox couple in the anodic region at E1/2 = 0.79 V vs. Ag/AgCl due to the RuIII/II couple. DNA titrations and ethidium bromide displacement experiments indicate the ruthenium porphyrin interacts with DNA potentially through a partial intercalation mechanism. Irradiation of aqueous solutions of the ruthenated complex and supercoiled DNA at a 100:1 base pair/complex ratio with visible light above 400 nm indicates that the complex causes double-strand breaks of the DNA.(© Wiley-VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2009)
Databáze: OpenAIRE
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