Hemoglobin Immobilization on Multiporous Nanofibers of SnO2 and Chitosan Composite for Hydrogen Peroxide Sensing
| Autor: | Samiul Alim, A.K.M. Kafi, Rajan Jose, Mashitah M. Yusoff |
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| Rok vydání: | 2019 |
| Předmět: |
Materials science
Biomedical Engineering Bioengineering 02 engineering and technology General Chemistry 021001 nanoscience & nanotechnology Condensed Matter Physics Redox Amperometry Electrospinning Electron transfer Chemical engineering Nanofiber Electrode General Materials Science Cyclic voltammetry 0210 nano-technology Biosensor |
| Zdroj: | Journal of Nanoscience and Nanotechnology. 19:2027-2033 |
| ISSN: | 1533-4880 |
| DOI: | 10.1166/jnn.2019.15465 |
| Popis: | A multiporous nanofiber (MPNFs) of SnO₂ and chitosan has been used for the immobilization of a redox protein, hemoglobin (Hb), onto the surface of glassy carbon electrode (GCE). The multiporous nanofiber of SnO₂ that has very high surface area is synthesized by using electrospinning technique through controlling the tin precursor concentration. Since the constructed MPNFs of SnO₂ exposes very high surface area, it increases the efficiency for biomolecule-loading. The morphology of fabricated electrodes is examined by SEM observation and the absorbance spectra of Hb/(MPNFs) of SnO₂ are studied by UV-Vis analysis. Cyclic Voltammetry and amperometry are employed to study and optimize the performance of the resulting fabricated electrode. After fabrication of the electrode with the Hb and MPNFs of SnO₂, a direct electron transfer between the protein's redox centre and the glassy carbon electrode was established. The modified electrode has showed a couple of redox peak located at -0.29 V and -0.18 V and found to be sensitive to H₂O₂. The fabricated electrode also exhibited an excellent electrocatalytic activity towards the reduction of H₂O₂. The catalysis currents increased linearly to the H₂O₂ concentration in a wide range of 5.0×10-6-1.5×10-4 M. Overall experimental results show that MPNFs of SnO₂ has a role towards the enhancement of the electroactivity of Hb at the electrode surface. Thus the MPNFs of SnO₂ is a very promising candidate for future biosensor applications. |
| Databáze: | OpenAIRE |
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