A Potential Capacitance Detection and Enumeration Method forVibrio choleraeandVibrio vulnificus

Autor:	R. M. Grodner, Douglas L. Marshall, David N. Domma
Rok vydání:	1999
Předmět:	Chromatography biology Vibrio vulnificus Aquatic Science biology.organism_classification medicine.disease_cause Capacitance Rapid detection Tryptic soy broth Microbiology Sodium salt chemistry.chemical_compound Test code chemistry Vibrio cholerae Enumeration medicine Food Science
Zdroj:	Journal of Aquatic Food Product Technology. 8:5-19
ISSN:	1547-0636 1049-8850
DOI:	10.1300/j030v08n01_03
Popis:	Salt content of basal modified cellobiose-polymyxin B-colistin (mCPC) medium was varied to assess impedance-based detection of Vibrio cholerae and Vibrio vulnificus. Several sodium salts were tested as substitutes for NaCl. Bactometer detection times (h) were compared to find the optimal salt and its concentration. Results using tryptic soy broth indicated 2% Na2HPO4 was the best NaCl replacement. Use of 2% Na2HPO4 with 0.5% NaCl in mCPC medium allowed rapid detection of both vibrios using a capacitance test code. Correlation of capacitance detection times with MPN/ml gave r = −0.74 and r2 = 0.54 for V. cholerae and r = −0.68 and r2 = 0.46 for V. vulnificus. With starting counts above 104 MPN/ml, both pathogens could be enumerated in less than 20 h with this method.
Databáze:	OpenAIRE
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