Identification of Chlamydia trachomatis proteins recognized by T cells from high-risk women resistant to reinfection and/or protected from ascending infection. (VAC7P.961)
| Autor: | Toni Darville, Xiaojing Zheng, Catherine O'Connell, Michele Picard, Harold Wiesenfeld, Sharon Hillier, Jessica Flechtner |
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| Rok vydání: | 2014 |
| Předmět: | |
| Zdroj: | The Journal of Immunology. 192:141.6-141.6 |
| ISSN: | 1550-6606 0022-1767 |
| DOI: | 10.4049/jimmunol.192.supp.141.6 |
| Popis: | Murine and human data indicate IFNγ-producing Chlamydia-responsive CD4 T cells are key to protection from genital tract disease, and chlamydial proteins have been identified that induce protection in mice. A preventative vaccine against C. trachomatis requires the identification of T cell antigens that induce protection in humans. We analyzed peripheral blood CD4 and CD8 T cell responses from high-risk women defined as “resistant” or “susceptible” based on negative evidence for reinfection or detection of repeated reinfection during a 12-month follow-up period. Autologous APCs preincubated with E. coli clones expressing individual chlamydial ORFs were coincubated with CD4 or CD8 T cells and IFN-γ measured by ELISA. CD4 T cells from resistant women recognized 16 proteins significantly more frequently when compared to susceptible women, and only 2 of 16 were among a subset of immunodominant CD4 proteins suggesting restricted MHC recognition. No proteins recognized by CD8 T cells met immunoprotective criteria. Five CD4 and 5 CD8 antigens were more frequently recognized by women who had cervical infection but no evidence of endometrial infection by PCR. Thus, although protection from reinfection appears dependent on CD4 T cells, both CD4 and CD8 T cells may contribute to disease prevention. The use of samples from persons with evidence of natural immune protection for antigen discovery should advance development of a vaccine against C. trachomatis. |
| Databáze: | OpenAIRE |
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