Rapid and Economical Colorimetric Detection of Cyanide in Blood Using Vitamin B12
| Autor: | Sardar Ali Wattoo, Muhammad Ashraf Tahir, Muhammad Imran, Muhammad Taimoor Chaudhary, Ammar M. Tahir, Muhammad Sarwar, Ghulam Mustafa, Adeel Subhani |
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| Rok vydání: | 2015 |
| Předmět: |
Detection limit
Chromatography Filter paper Thiocyanate Chemistry Cyanide 010401 analytical chemistry Inorganic chemistry Hydrogen cyanide 02 engineering and technology 021001 nanoscience & nanotechnology 01 natural sciences 0104 chemical sciences Pathology and Forensic Medicine chemistry.chemical_compound Arsine Vitamin B12 0210 nano-technology Carbon monoxide |
| Zdroj: | Australian Journal of Forensic Sciences. 48:42-49 |
| ISSN: | 1834-562X 0045-0618 |
| DOI: | 10.1080/00450618.2015.1025840 |
| Popis: | This work was aimed at the development and validation of an economical colorimetric method for detection of cyanide in whole blood. The method was based on the reaction of hydrogen cyanide with vitamin B12. The hydrogen cyanide is released on acidification of the blood sample. The vitamin B12 was loaded on a strip of a commonly used filter paper by impregnating the strip in a 1.0% (w/v) solution of vitamin B12 under alkaline conditions. The limit of detection of this method was found to be 1.0 mg L−1 which is lower than the reported lethal blood concentration of cyanide (2.5 mg L−1). The method was free from interferences from the common gases including ammonia, carbon dioxide, arsine, phosphine and carbon monoxide, thiocyanate, organophosphate pesticides and nitrile-containing pesticides. This method was successfully employed for detection of cyanide in several cases reported to the forensic toxicology. |
| Databáze: | OpenAIRE |
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