Autor:	S. Ismail, R. V. Sairam, K. Berry, R. Hassel, Gregory Franklin, B. Smith, K. Meeker, Stephen L. Goldman, Nilesh D. Kashikar, D. Al Abed, Madasamy Parani
Rok vydání:	2003
Předmět:	Regeneration (biology) fungi food and beverages Plant physiology Organogenesis Horticulture Biology chemistry.chemical_compound chemistry Callus Shoot Botany Sorbitol Cultivar Explant culture
Zdroj:	Plant Cell, Tissue and Organ Culture. 75:79-85
ISSN:	0167-6857
Popis:	We have developed an efficient protocol for callus induction and plant regeneration in three elite soybean cultivars (Williams 82, Loda and Newton). The technique is most novel in that the shoot buds developed from the nodal callus. Callus induction and subsequent shoot bud differentiation were achieved from the proximal end of cotyledonary explants on modified Murashige and Skoog (MS) media containing 2.26 μM 2,4-dichlorophenoxy-acetic acid (2,4-D) and 8.8 μM benzyladenine (BAP), respectively. Varying the carbon source optimized the regeneration system further. Among the various carbon sources tested, sorbitol was found to be the best for callus induction and maltose for plant regeneration.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::bc9909aba45ca03d0ac21131f8f1d51d https://doi.org/10.1023/a:1024649122748 Zobrazit plný text záznamu Full text from SpringerLink