Post-thaw Thermal Resistance Test on Motility and Acrosomal Integrity of Filtered and Non-filtered Frozen Semen of Murrah Buffalo Bulls

Autor:	R. K. Tuli, V. P. Maurya
Rok vydání:	2003
Předmět:	endocrine system food.ingredient biology urogenital system Chemistry Extender Semen biology.organism_classification Sperm law.invention Murrah buffalo Andrology food Sephadex law Yolk Animal Science and Zoology Acrosome Sperm motility Food Science
Zdroj:	Asian-Australasian Journal of Animal Sciences. 16:1424-1428
ISSN:	1976-5517 1011-2367
DOI:	10.5713/ajas.2003.1424
Popis:	Present investigation was conducted to determine the post-thaw sperm motility and acrosomal damage of filtered and non-filtered frozen semen of Murrah buffalo bulls. Twenty semen ejaculates (from four Murrah buffalo bulls collected at weekly interval) were diluted in Tris egg yolk glycerol extender and divided into two parts. One was filtered through sephadex G-100 column and the other portion was kept as such (non-filtered). Both fractions were frozen in liquid nitrogen (-196°C) by the standard method developed in the laboratory. After 24 h of freezing, non-filtered and filtered semen samples were thawed at 37°C for 1 min. These samples were incubated at 37°C in a water both. The different seminal characteristics i.e. percent progressive sperm motility, live and abnormal spermatozoa and spermatozoa with damaged acrosome were assessed at hourly interval till they remained motile. The filtered frozen and thawed semen showed significantly (p
Databáze:	OpenAIRE
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