Biocatalysts Based on Bacterial Cells with Amidase Activity for the Synthesis of Acrylic Acid from Acrylamide
| Autor: | Yu. G. Maksimova, A. Yu. Maksimov, M. S. Yakimova |
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| Rok vydání: | 2019 |
| Předmět: |
Chromatography
Alcaligenes faecalis biology 010405 organic chemistry Substrate (chemistry) biology.organism_classification 01 natural sciences Catalysis 010406 physical chemistry 0104 chemical sciences Chitosan chemistry.chemical_compound chemistry Biocatalysis Acrylamide Amidase activity Glutaraldehyde Acrylic acid |
| Zdroj: | Catalysis in Industry. 11:264-270 |
| ISSN: | 2070-0555 2070-0504 |
| DOI: | 10.1134/s2070050419030073 |
| Popis: | The biocatalytic synthesis of acrylic acid from acrylamide by the Rhodococcus erythropolis 4-1 and Alcaligenes faecalis 2 strains with amidase activity is studied. The optimum pH values are 6–7 for R. erythropolis 4-1 and 7–7.5 for A. faecalis 2, while the optimum temperature is 20–50°С for both strains. The optimum acrylamide concentration is 150 mM for R. erythropolis 4-1 and 250 mM for A. faecalis 2. The synthesis of acrylic acid with fractional additions of a substrate catalyzed by the biomass of A. faecalis 2 is more effective than using R. erythropolis 4-1. The biocatalyst is best stored at −20°C. The amidase activity of A. faecalis 2 cells immobilized on chitosan activated with glutaraldehyde and non-activated chitosan did not decline during the storage of either wet or dried granules. |
| Databáze: | OpenAIRE |
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