Distinct roles of two eIF4E isoforms in the germline ofCaenorhabditis elegans
| Autor: | Melissa A. Henderson, Hayden P. Huggins, David S. Buckner, Peter R. Boag, Madhu S. Sengupta, Jenna L. Hoffman, Myon-Hee Lee, Brett D. Keiper, Hamilton J. Stoffel, Jacob S. Subash |
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| Rok vydání: | 2020 |
| Předmět: |
Gene isoform
0303 health sciences integumentary system biology EIF4E Translation (biology) Cell Biology Cell fate determination biology.organism_classification Oocyte Germline Cell biology 03 medical and health sciences 0302 clinical medicine medicine.anatomical_structure Polysome medicine 030217 neurology & neurosurgery Caenorhabditis elegans 030304 developmental biology |
| Zdroj: | Journal of Cell Science. |
| ISSN: | 1477-9137 0021-9533 |
| DOI: | 10.1242/jcs.237990 |
| Popis: | Germ cells use both positive and negative mRNA translational control to regulate gene expression that drives their differentiation into gametes. mRNA translational control is mediated by RNA-binding proteins, miRNAs, and translation initiation factors. We have uncovered the discrete roles of two translation initiation factor eIF4E isoforms (IFE-1 and -3) that bind m7GTP mRNA caps during C. elegans germline development. IFE-3 plays important roles in germline sex determination (GSD), where it promotes oocyte cell fate and is dispensable for spermatogenesis. IFE-3 is expressed throughout the germline, localizes to germ granules but distinct from IFE-1 and PGL-1, and facilitates oocyte growth and viability. This contrasts with the robust expression of IFE-1 in spermatocytes, the isoform that resides within P granules in spermatocytes and oocytes, and promotes late spermatogenesis. Each eIF4E is localized by its cognate eIF4E binding protein (IFE-1:PGL-1 and IFE-3:IFET-1). IFE-3 and IFET-1 regulate translation of several GSD mRNAs, but not those under control of IFE-1. Distinct mutant phenotypes, in vivo localization, and differential mRNA translation suggest independent dormant and active periods for each eIF4E isoform in the germline. |
| Databáze: | OpenAIRE |
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