HPLC-UV analytical method for determination of pizotifen after in vitro transdermal diffusion studies
| Autor: | M.A. Calatayud-Pascual, S. del Rio Sancho, C.E. Serna-Jiménez, A. Femenía-Font, C. Balaguer-Fernández, Virginia Merino, Alicia López-Castellano |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Pharmacology
Detection limit Chromatography Chemistry Diffusion Clinical Biochemistry General Medicine Pizotifen Biochemistry High-performance liquid chromatography In vitro Analytical Chemistry Serotonin inhibitor Drug Discovery medicine Molecular Biology Quantitative analysis (chemistry) medicine.drug Transdermal |
| Zdroj: | Biomedical Chromatography. 26:769-774 |
| ISSN: | 0269-3879 |
| DOI: | 10.1002/bmc.1727 |
| Popis: | Pizotifen malate is an antihistamine and serotonin inhibitor used in the preventive treatment of migraine and eating disorders. A simple, rapid, accurate and precise high-performance liquid chromatography (HPLC) method involving ultraviolet detection was validated for the quantitative analysis of pizotifen malate in samples from in vitro transdermal diffusion studies. The method was validated for specificity, linearity, accuracy, precision, limit of detection, limit of quantification and robustness. Drug stability in the solution was also determined under different conditions. Separation was carried out using a 250 × 4.0 mm Kromasil(®) C(18) column at room temperature. The detector response, fitted at 254 nm, was found to be linear in a concentration range between 0.24 and 24.0 µg/mL. The limit of detection was 0.02 µg/mL and the limit of quantification was 0.07 µg/mL. Finally, in vitro transdermal diffusion of pizotifen malate was characterized using the validated HPLC method. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text