Genotypic diversity and distribution ofSphaeropsis sapineawithinPinus radiatatrees from northern Spain
| Autor: | Ana Aragonés, Tania Manzanos, Eugenia Iturritxa |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine education.field_of_study Genetic diversity Ecology Pinus radiata Population Forestry 030108 mycology & parasitology Biology biology.organism_classification 01 natural sciences 03 medical and health sciences Genetic marker Genetic structure Botany Genetic variation Microsatellite Genetic variability education human activities 010606 plant biology & botany |
| Zdroj: | Forest Pathology. 49:e12550 |
| ISSN: | 1437-4781 |
| DOI: | 10.1111/efp.12550 |
| Popis: | Sphaeropsis sapinea is an important latent pathogen of Pinus spp., outbreaks of which have a considerable impact on plantations. This study considers the population diversity and distribution of S. sapinea in northern Spain at different spatial scales from single plantations to a wide area covered by Pinus radiata trees. Estimation of genotypic diversity is an important component of the analysis of the genetic structure of plant pathogen populations. Ten simple sequence repeat (SSR) markers were used, together with vegetative compatibility tests, to study the genetic diversity among S. sapinea isolates. Polymorphism analysis at SSR loci is a simple and direct approach for estimating the genetic diversity of S. sapinea isolates. From a total of 86 isolates collected from four different areas, 14 microsatellite haplotypes and 13 vegetative compatibility groups (VCGs) were identified. The percentage of maximum genotypic diversity, based on Stoddart and Taylor's index, for microsatellites of the northern Spain population ranged from 14.6% to 38.1% and from 8.0% to 29.4% for VCGs. Analysis of these markers and vegetative compatibility groups confirmed that S. sapinea reproduces mainly asexually due to its reduced genotypic diversity in spatially close populations. Isolates of S. sapinea from northern Spain populations were predominantly monomorphic at the tested SSR loci. Vegetative compatibility groups also indicate a low level of genetic variability in these samples, which appear to be clonal. |
| Databáze: | OpenAIRE |
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