| Autor: | Iain B. H. Wilson, Erika Staudacher, Friedrich Altmann, Leopold März |
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| Rok vydání: | 1999 |
| Předmět: | |
| Zdroj: | Glycoconjugate Journal. 16:109-123 |
| ISSN: | 0282-0080 |
| DOI: | 10.1023/a:1026488408951 |
| Popis: | Baculovirus-mediated expression in insect cells has become well-established for the production of recombinant glycoproteins. Its frequent use arises from the relative ease and speed with which a heterologous protein can be expressed on the laboratory scale and the high chance of obtaining a biologically active protein. In addition to Spodoptera frugiperda Sf9 cells, which are probably the most widely used insect cell line, other mainly lepidopteran cell lines are exploited for protein expression. Recombinant baculovirus is the usual vector for the expression of foreign genes but stable transfection of - especially dipteran - insect cells presents an interesting alternative. Insect cells can be grown on serum free media which is an advantage in terms of costs as well as of biosafety. For large scale culture, conditions have been developed which meet the special requirements of insect cells. |
| Databáze: | OpenAIRE |
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