Factor XIII A is synthesized and expressed on the surface of U937 cells and alveolar macrophages
| Autor: | Garry W. Lynch, Richard L. Kradin, Robert B. Colvin, Jan McDonagh, James T. Kurnick, Mark Erikson |
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| Rok vydání: | 1987 |
| Předmět: | |
| Zdroj: | Blood. 69:778-785 |
| ISSN: | 1528-0020 0006-4971 |
| DOI: | 10.1182/blood.v69.3.778.778 |
| Popis: | Factor XIII A subunit was detected in U937 cells and human alveolar macrophages by immunohistology and Western blotting. U937 cells synthesize factor XIII A subunit de novo under serum-free, platelet- free conditions, as indicated by 35S-methionine labeling and immunoprecipitation. Thrombin-dependent activity was demonstrated to account for 98% of the total transglutaminase activity in U937 cells (1.5 micrograms per 0.5 X 10(6) cells/mL). Intact U937 cells and alveolar macrophages and homogenates from these cells cross-linked fibrin to form gamma-gamma and alpha-polymers. Factor XIII A was detected on the surface of intact U937 cells and macrophages by flow cytometry and 125I-labeling and immunoprecipitation. Cell surface expression of factor XIII A was augmented in the presence of several soluble macrophage activators; however, no concurrent increase in its biosynthesis was observed. The presence and cell surface expression of factor XIII A subunit within macrophages suggest new pathways by which these cells may function in clotting and in the remodeling of the extracellular matrix during inflammation and wound healing. |
| Databáze: | OpenAIRE |
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