A microfabricated quantum dot-linked immuno-diagnostic assay (μQLIDA) with an electrohydrodynamic mixing element
| Autor: | Peter Clark, Gi-Beum Kim, Chengjie Yu, Moses Noh, Leonid Zubkov, Elisabeth S. Papazoglou |
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| Rok vydání: | 2015 |
| Předmět: |
Microchannel
Chromatography biology Chemistry Microfluidics Metals and Alloys Nanotechnology Condensed Matter Physics Surfaces Coatings and Films Electronic Optical and Magnetic Materials Quantum dot Myeloperoxidase Materials Chemistry biology.protein Electrohydrodynamics Electrical and Electronic Engineering Inflammatory biomarker Instrumentation Biosensor Mixing (physics) |
| Zdroj: | Sensors and Actuators B: Chemical. 209:722-728 |
| ISSN: | 0925-4005 |
| DOI: | 10.1016/j.snb.2014.12.033 |
| Popis: | We present a microfabricated quantum dot-linked immuno-diagnostic assay (μQLIDA) biosensor with an embedded mixing element. We adapted our previous capillary-based system into a microfabricated planar microchannel, which has been integrated with an active, electrothermal mixing component. With the active mixing element, the detection time was significantly reduced from about 3.5 h to 30 min in a case study of detecting myeloperoxidase (MPO). MPO is an inflammatory biomarker that is over-expressed in inflammatory conditions including inflammatory bowel disease and cardiovascular disease. The μQLIDA biosensor presented here is a low-cost, rapid immunofluorescence sensor that can detect nanomolar concentrations of MPO, with a working volume of 2 μl. |
| Databáze: | OpenAIRE |
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