Isolation and characterization of polymorphic microsatellite markers from European pear (Pyrus communis L.)

Autor:	N. G. Harvey, C. M. James, Felicidad Fernández-Fernández
Rok vydání:	2006
Předmět:	Genetics PEAR Malus Ecology Locus (genetics) Biology biology.organism_classification Biochemistry General Biochemistry Genetics and Molecular Biology genomic DNA Polymorphic Microsatellite Marker Microsatellite Genomic library Pyrus communis
Zdroj:	Molecular Ecology Notes. 6:1039-1041
ISSN:	1471-8286 1471-8278
DOI:	10.1111/j.1471-8286.2006.01422.x
Popis:	This study reports the development and characterization of 19 microsatellite primer pairs developed from genomic DNA of European pear (Pyrus communis) and their transferability to other Pyrus and Malus material. The primers were designed from two different genomic libraries enriched for di- and trinucleotide repeats. When tested in six P. communis cultivars and 15 other Pyrus species, 13 primers revealed single-locus polymorphism and six showed more complex patterns that suggest multiple loci. Two to 18 alleles were detected per locus and two primer pairs were sufficient to discriminate all accessions. Transferability of nine primer pairs to Malus was demonstrated through amplification of discrete products in two accessions.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::bc238813fd7ef68d5d8912c7b2daff61 https://doi.org/10.1111/j.1471-8286.2006.01422.x Zobrazit plný text záznamu Plný text