Enantiomeric separation and optical purity determination of thyroxine enantiomers in bulk and pharmaceutical formulations

Autor:	Hassan Y. Aboul-Enein, Laila I. Abou-Basha
Rok vydání:	1995
Předmět:	Detection limit chemistry.chemical_compound Chromatography Chemistry Calibration curve Molecular Medicine Methanol General Pharmacology Toxicology and Pharmaceutics Enantiomer Enantiomeric excess Acetonitrile High-performance liquid chromatography Dosage form
Zdroj:	Pharmaceutica Acta Helvetiae. 70:237-240
ISSN:	0031-6865
DOI:	10.1016/0031-6865(95)00024-4
Popis:	A base-line separation of racemic thyroxine is achieved by an HPLC method using an ovomucoid protein column. The mobile phase consists of 20 mM KH2PO4 (pH 6.7)/acetonitrile/methanol (100:15:5), and the effluent is monitored by UV detection at 220 nm. A standard linear calibration curve is constructed over the range of 0.25–10 nmol l -thyroxine sodium (L-T4) for the determination of optical purity of L-T4 in pharmaceutical formulations. The method is specific and sufficiently sensitive with a lower detection limit of 0.1 nmol (for both L-T4 and D-T4). Data demonstrating recovery and precision of the assay is presented, and the method is applied to determine l -thyroxine sodium in injectable formulations.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::bbfb6f34072966380ae21f1dc3dec853 https://doi.org/10.1016/0031-6865(95)00024-4 Zobrazit plný text záznamu Full Text from ScienceDirect