Cloning, nucleotide sequence determination and expression of the hyaluronate lyase gene

Autor: Keith T. Holland, David Taylor, Angela M. Farrell
Rok vydání: 1995
Předmět:
Zdroj: FEMS Microbiology Letters. 130:81-85
ISSN: 0378-1097
DOI: 10.1016/0378-1097(95)00187-a
Popis: The Staphylococcus aureus 8325-4 hyaluronate lyase gene (hysA) was identified after detecting hyaluronate lyase activity expressed by phages from a genomic library. The hysA open reading frame, capable of encoding a protein of 91 980 Da, was identified by Tn 5 mutagenesis and nucleotide sequencing. HysA shares 35 and 36% amino acid sequence identity with group B streptococcal hyaluronate lyase and pneumococcal hyaluronidase, respectively. A 94-kDa protein was expressed in Escherichia coli minicells, a result consistent with the coding capacity of hysA. Identification of the S. aureus 8325-4 hyaluronate lyase gene will allow the regulation of this putative virulence determinant to be studied. The nucleotide sequence data have been deposited with Genbank, accession number U21221.
Databáze: OpenAIRE