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The aim of this greenhouse experiment was the assessment of the influence of H 2 SeO 3 at soil concentrations of 0.05, 0.15 and 0.45 mmol kg -1 , on the activity of selected oxidoreductive enzymes in wheat (Triticum aestivum), The wheat plants were grown in 2dm 3 pots filled with dust-silt black soil of pH 7.7. Applied H 2 SeO 3 caused activation of plant nitrate reductase at all concentrations, but activation of plant polyphenol oxidase at only two lower concentrations. The highest concentration caused inhibition of polyphenol oxidase and peroxidase. Plant catalase activity decreased under the influence of 0.15 and 0.45 mmol kg -1 concentration. After the final analysis Se was quantified in plants and soil. The amounts in plants were: control (unamended soil) 1.95 mg kg -1 ; I dose (0.05 mmol kg -1 ) 18.27 mg kg -1 ; II dose (0.15 mmol kg -1 ) 33.20 mg kg -1 and III dose (0.45 nmol kg -1 ) 38.37 mg kg -1 , in soil: 0.265 mg kg -1 ; 3.61 mg kg -1 ; 10.53 mg kg -1 ; 30.53 mg kg -1 ; respectively. Simultaneously, a laboratory experiment was performed, where the activity of soil catalase and peroxidase were tested after 1, 3, 7, 14, 28, 56, and 112 days after Se treatment. Peroxidase activity in soil decreased with increasing Se content, over the whole experiment. The lowest dose of Se caused activation a significant 10% increase in catalase activity, but the influence of others doses was unclear. |
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