Expression of the 2,4-D degrading plasmid pJP4 ofAlcaligenes eutrophus inRhizobium trifolii

Autor:	E. Gartner, L. Feng, Ivan R. Kennedy, B. G. Rolfe, L. Van Zwieten
Rok vydání:	1994
Předmět:	Rhizobiaceae Strain (chemistry) Inoculation food and beverages Bioengineering Cometabolism Biology Biodegradation biology.organism_classification Applied Microbiology and Biotechnology Microbiology chemistry.chemical_compound Plasmid chemistry Nitrogen fixation Bacteria Biotechnology
Zdroj:	Acta Biotechnologica. 14:119-129
ISSN:	1521-3846 0138-4988
DOI:	10.1002/abio.370140202
Popis:	The 2,4-dichlorophenoxy acetic acid (2,4-D) degrading plasmid, pJP4, was transferred into Rhizobium trifolii ANU843 from its nature host Alcaligenes eutrophus JMP134 by conjugation. The ability to degrade 2,4-D was expressed in the transconjugant ANU843p as shown by a total loss of UV-absorbent compounds and by gas chromatographic analysis. However, the transconjugant was unable to grow on 2,4-D alone. When the transconjugant strain ANU843p was inoculated onto white and subterranean clover plants in laboratory trials, the transconjugant retained the capacity of nodulation, but the nitrogen-fixation activity was diminished, particularly in the case of subterranean clover. The plasmid in the transconjugant was stable in nodules for at least nine weeks after inoculation and could be of value in applications requiring the protection or removal of the 2,4-D involving cometabolism with plant substrates.
Databáze:	OpenAIRE
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