Nitrate (13NO3−) flux studies and response to tungstate treatments in wild type barley and in an NR‐deficient mutant

Autor:	J. Wieneke
Rok vydání:	1994
Předmět:	inorganic chemicals Phenylglyoxal Physiology Chemistry Mutant Wild type food and beverages Root system Nitrate reductase Horticulture chemistry.chemical_compound Botany Shoot Poaceae Hordeum vulgare Agronomy and Crop Science
Zdroj:	Journal of Plant Nutrition. 17:127-146
ISSN:	1532-4087 0190-4167
DOI:	10.1080/01904169409364714
Popis:	Hydroponic experiments were conducted with seedlings of barley (Hordeum vulgare L.), comparing the wild type and an NR‐deficient mutant (AZ 12/70), to monitor the NO3 − influx/efflux relations and the response to tungstate treatment using 13N‐labelling. Upon treatment with the arginyl‐residue‐binding inhibitor phenylglyoxal both genotypes responded with an immediate depression of NO3 − influx. At low external NO3 − concentrations efflux was small (5–15% of the influx) and did not differ substantially between the two genotypes. Although the mutant was distinguished from the wild type by a thinner root system and a reduced shoot length, the total N distribution between roots and shoots and the N concentrations in the root and shoot tissues were fairly comparable. Substantially higher extractable NO3 − concentration in the shoots (but not roots) of the mutant may indicate that the capacity to reduce NO3 − was restricted due to the very low but still detectable NR activity in the root and shoot tissu...
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::bbd9f4df9c1eff8265fbe88a33feec30 https://doi.org/10.1080/01904169409364714 Zobrazit plný text záznamu