Determination of Candesartan and Hydrochlorothiazide in Human Plasma by HPLC Coupled with Mass Spectrometry

Autor:	Roman V. Gurto, Elena A. Yanovskaya, Natalia U. Polomeeva, M. S. Timofeev, V. V. Udut, Olga S. Brushinina, Galina A. Frelikh, Yulya G. Zyuz’kova, Vladimir A. Slepichev
Rok vydání:	2014
Předmět:	Electrospray Candesartan Chromatography Hydrochlorothiazide Pharmacokinetics Liquid chromatography–mass spectrometry Chemistry Analytical chemistry medicine Atmospheric-pressure chemical ionization Mass spectrometry High-performance liquid chromatography medicine.drug
Zdroj:	International Journal of Analytical Mass Spectrometry and Chromatography. :25-32
ISSN:	2332-1776 2332-1768
DOI:	10.4236/ijamsc.2014.22003
Popis:	Quantitative determination of hydrochlorothiazide (HCTZ) and candesartan (CDS) in human plasma in volunteers was performed using a sensitive, selective and specific LC-MS method which has been developed and validated before the study. The study was performed by means of a liquid chromatograph Shimadzu Prominence equipped with a mass spectrometer LCMS-2020. Analytical column PerfectBond ODS-HD HPLC-column 5 μm 250 × 3.0 mm with a pre-column cartridge PerfectBond ODS-HD 5 μm 10 × 3.0 mm, double source of ionization for LCMS-2020 (electrospray (ESI) and chemical (APCI)) and software LabSol LCMS V5 LCMS2020 systempack were used. The low limit of the quantitative determination for HCTZ and CDS made up 10 ng/ml. m/z for CDS 441.20—positive scan, m/z for HCTZ 295.90—negative scan. The method has been applied to a pharmacokinetic study of 12.5 mg HCTZ and 16 mg CDS tablet in healthy volunteers.
Databáze:	OpenAIRE
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