Characterization of lymphoid DC during the resolution of Listeria monocytogenes infection (43.38)
| Autor: | Bryan Michael Burt, George Plitas, Hoang Nguyen, Jennifer Stableford, Ronald P. DeMatteo |
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| Rok vydání: | 2007 |
| Předmět: | |
| Zdroj: | The Journal of Immunology. 178:S44-S44 |
| ISSN: | 1550-6606 0022-1767 |
| DOI: | 10.4049/jimmunol.178.supp.43.38 |
| Popis: | Introduction: Dendritic cells are critical to innate immune responses based on their ability to secrete inflammatory cytokines and prime naïve T-cells. The role of DC during later stages of infection is not known. Methods: Lymphoid DC (CD11c+CD8+NK1.1−B220−) were analyzed by flow cytometry and isolated by fluorescence activated cell sorting from the spleens of C57BL/6 mice infected with 5000 CFU of LM strain 10403s by tail vein injection. Mixed leukocyte reaction (MLR) was performed with 1x105 allogeneic T cells and 3x104 lymphoid DC. Results: Following 24 hours of infection lymphoid DC accounted for 11% of conventional DC (CD11c+B220−NK1.1−) and had high expression of MHC II, CD40 and CD86. After 7 days of infection lymphoid DC increased to 47% of conventional DC and had less MHC II, CD40 and CD86 expression than uninfected controls. Lymphoid DC also lost their ability to stimulate allogeneic T cells in the MLR (6634±466 CPM for 12 hours of infection vs 320±80 for 7 days of infection). Conclusion: Dynamic changes occur in the lymphoid DC compartment during the resolution phase of LM infection. This work was supported by grant DK068346. |
| Databáze: | OpenAIRE |
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