Recovery of mangostins from Garcinia mangostana peels with an aqueous micellar biphasic system

Autor: Ka-Heng Lee, John Chi-Wei Lan, Hui Suan Ng, Wolfgang Zimmermann, Hip Seng Yim, Grace Yin Tze Tan
Rok vydání: 2017
Předmět:
Zdroj: Food and Bioproducts Processing. 102:233-240
ISSN: 0960-3085
Popis: Methods of extraction and separation of antioxidants from plant sources by chromatography and solvent extraction are often time and solvent consuming, and result in low yields. A simple and mild thermo-induced aqueous micellar biphasic system (AMBS) was used to recover α- and γ-mangostins from Garcinia managostana peels. AMBS composed of non-ionic surfactants such as Triton X-114, Triton X-45, and Pluronic triblock copolymers were developed. Effects of the types of the non-ionic surfactant, its concentration, sample load, pH of the AMBS and the equilibration temperature on recovery of mangostins were studied. The optimum condition for recovery of α- and γ-mangostins was achieved with an AMBS composed of 3% (w/w) Pluronic L-81 and a sample loading of 0.5% (w/w) crude peels. At equilibration temperature of 40 °C and pH 8, 2646.6 ± 1.2 μg/mL of α-mangostin and 379.4 ± 1.2 μg/mL of γ-mangostin were recovered in the micelle-rich phase of the AMBS with a log partition coefficient of α- (log K α ) and γ-mangostin (log K γ ) of 4.00 ± 0.02 and 3.36 ± 0.01, respectively. α- and γ-mangostins could be recovered simultaneously with a yield of 52.93% and 7.59%, respectively in a single-step. This study demonstrated that AMBS can serve as a tool to efficiently recover and separate various bioactive compounds from plant sources.
Databáze: OpenAIRE
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