Characterizing the Interference between Two Comoviruses in Cowpea
| Autor: | Kenneth C. Eastwell, Gabriel B. Kalmar |
|---|---|
| Rok vydání: | 1997 |
| Předmět: | |
| Zdroj: | Journal of the American Society for Horticultural Science. 122:163-168 |
| ISSN: | 2327-9788 0003-1062 |
| DOI: | 10.21273/jashs.122.2.163 |
| Popis: | In certain cultivars of cowpea [Vigna unguiculata (L.) Walp.] that are operationally immune to cowpea mosaic virus strain SB (CPMV), coinoculation of CPMV with cowpea severe mosaic virus strain DG (CPSMV) reduces severity and delays expression of symptoms normally induced by CPSMV alone. In cultivars susceptible to both viruses, coinoculation delays development of symptoms in response to CPSMV. Using monoclonal antibodies for serological assays and virus-specific RNA probes for hybridization, it is demonstrated that the presence of CPMV in the inoculum yields a concomitant delay in the synthesis of CPSMV coat protein and replication of CPSMV RNA and restricts the transport of CPSMV out of infection centres. Only bottom component of CPMV containing RNA1 is required to offer protection against CPSMV. Destroying the integrity of CPMV RNA eliminates its protective capability. In cowpea cultivars that are operationally immune to CPMV, the presence of CPSMV in the inoculum is unable to compensate for events of CPMV replication that are inhibited. The lack of complementation suggests a high degree of specificity in the replication of these two comoviruses. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|