Effect of the expression of the human pub gene on the proliferation and differentiation of rat pheochromocytoma PC-12 cells

Autor: E. L. Arsen’eva, E. S. Manuilova, Igor A. Grivennikov, E. V. Novosadova, N. V. Khaidarova, Vyacheslav Z. Tarantul, A. N. Lebedev, A. G. Kobylyanskii
Rok vydání: 2011
Předmět:
Zdroj: Neurochemical Journal. 5:69-72
ISSN: 1819-7132
1819-7124
Popis: We studied the effect of overexpression of the human pub gene on the proliferation and neuronal differentiation of PC-12 cells treated with nerve growth factor. We obtained a stable cell line overexpressing the human pub gene (the PC12-hpub line) and PC12-DNA3 control cell line. Using reverse transcription PCR, we showed transgene expression in cells of the PC12-hpub line. We found that under standard culturing conditions and under conditions of deprivation of trophic factors (1% embryonic calf serum), the growth rate of PC12-hpub cells did not differ from the rate of control PC12-DNA3 cells. After the induction of differentiation in these cells lines using nerve growth factor in the absence of serum, we found that, in 5 days, the number of differentiated cells with the neuronal phenotype in the PC12-hpub cells was smaller by a factor of 5 as compared to the control PC12-DNA3 cells. Thus, overexpression of the human pub gene in rat pheochromocytoma PC12 cells led to the suppression of their nerve-growth-factor-induced differentiation.
Databáze: OpenAIRE
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