Effect of Inhibitors on Azo-Reduction of 4′-Dimethylaminoazobenzene-2-carboxylic Acid byClostridiumDiaphorase

Autor:	Chizuko Yatome, Kazuhiko Fujimi, Toshihiko Ogawa
Rok vydání:	1992
Předmět:	chemistry.chemical_classification Azo compound Tertiary amine biology Stereochemistry Carboxylic acid General Chemistry biology.organism_classification chemistry.chemical_compound Enzyme Clostridium chemistry Diaphorase Bacteria Benzoic acid
Zdroj:	Bulletin of the Chemical Society of Japan. 65:2349-2351
ISSN:	1348-0634 0009-2673
DOI:	10.1246/bcsj.65.2349
Popis:	The effect of inhibitors on the azo-reduction of 4′-dimethylaminoazobenzene-2-carboxylic acid (DMABC) by Clostridium diaphorase together with β-nicotinamide adenine dinucleotide, reduced form (NADH) has been investigated. The reduction was inhibited by o-aminobenzoic acid which is a reduction product of DMABC. Lineweaver–Burk plots indicated that o-aminobenzoic acid was a competitive inhibitor with respect to DMABC (Ki = 10.5 × 10−3 mol dm−3). Various monosubstituted benzoic acid derivatives were also competitive inhibitors, among which o-hydroxybenzoic acid was most effective.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::bba57bba9723ffb4c473536a7bb4c245 https://doi.org/10.1246/bcsj.65.2349 Zobrazit plný text záznamu