PCR-analysis of photoperiod sensitivity genes in bread wheat varieties from Bilatserkovska experimental breeding station
| Autor: | A. A. Bakuma, V. M. Filimonov, L. A. Burdenyuk-Tarasevich, S. V. Chebotar, G. A. Chebotar |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine photoperiodism hemic and immune systems chemical and pharmacologic phenomena Biology bacterial infections and mycoses complex mixtures 01 natural sciences respiratory tract diseases 03 medical and health sciences Horticulture 030104 developmental biology Sensitivity (control systems) Pcr analysis Gene 010606 plant biology & botany |
| Zdroj: | Visnik ukrains'kogo tovaristva genetikiv i selekcioneriv. 16:217-226 |
| ISSN: | 2415-3680 1810-7834 |
| DOI: | 10.7124/visnyk.utgis.16.2.1060 |
| Popis: | The aim. Determination of alleles of the photoperiod sensitivity genes Ppd-1 in 16 winter wheat varieties of the Bilatserkovska Experimental Breeding Station and identification of the haplotypes of Ppd-D1 gene according to the accepted classification. Methods. DNA isolation, allele-specific and nested PCR, electrophoresis in agarose and polyacrylamide gels, determination of the significant differences in the time of heading. Results. Plants of the varieties Vodohrai bilotserkivs’kiy; Bilotserkivs’ka napivkarlykova, Olesia, Perlyna lisostepu, Elehiia, Yasochka, Lybid’, Tsarivna, Lisova pisnia, Romantyka, Vidrada, Schedra nyva, Charodiika bilotsekivs’ka, Russa, Driada 1 have the genotype PpdA1b Ppd-B1b Ppd-D1a and belong to the VII haplotype of the Ppd-D1 gene. The genotype of the variety Legenda bilotsekivs’ka is characterized by the presence of the alleles Ppd-A1b Ppd-B1b Ppd-D1b and belongs to the IV haplotype of the Ppd-D1 gene. The significant differences (P = 0.01) in the time of heading was detected only between the varieties Russa and Legenda bilotsekivs’ka and was 12.4 days. Conclusions. Most BEBS varieties are characterized by genotype — PpdA1b Ppd-B1b Ppd-D1a, in which the allele Ppd-D1a determines insensitivity to the photoperiod and leads to early heading. Only variety Legenda bilotsekivs’ka is the carrier of the recessive allele of Ppd-D1 gene, belongs to the IV haplotype, has the latest time of heading. Keywords: Ppd-A1, Ppd-B1, Ppd-D1 genes, winter wheat, PCR analysis, photoperiod sensitivity. |
| Databáze: | OpenAIRE |
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