| Autor: |
Kiyohito Nagai, Masahiko Awaji, Yasunori Iwahashi, Takashi Yamamoto, Shugo Watabe, Shigeharu Kinoshita, Kaoru Maeyama, Fumihiro Hattori, Makoto Kakinuma |
| Rok vydání: |
2018 |
| Předmět: |
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| Zdroj: |
Biomineralization ISBN: 9789811310010 |
| Popis: |
In the current method of pearl production, the mantle fragment of a donor pearl oyster is transplanted into a host pearl oyster together with an inorganic bead (pearl nucleus). After this surgical procedure, only outer epithelial cells (OEC) in the transplanted mantle survive in a host pearl oyster and form a pearl sac to begin pearl formation. Therefore, implantation of only the OEC instead of the mantle fragment would be a possible alternative to the current procedure. To examine the potential of pearl production by implanting OEC in Pinctada fucata, we developed a cell implantation method using the pearl nucleus carrying a small pit inoculated with OEC. As a result, approximately 70% of the inserted nuclei formed the nacreous layer when the OEC were inoculated at 5 × 104 cells/nucleus. Then, OEC isolated from two genetically different types of pearl oysters that significantly differed in shell nacre color (yellowness) were mixed at four different ratios, and the prepared OEC mixtures were transplanted to investigate the effects of the blend on the yellowness of pearls to be harvested. The yellowness of harvested pearls differed significantly in accordance with the mixing ratio. Similarly, OEC isolated from two types of pearl oysters that showed a significant difference in the thickness of their shell nacre aragonite tablets were mixed at four different ratios and transplanted. Mean thickness of the aragonite tablets of the harvested pearls differed according to mixing ratio. These results suggest the method to control pearl quality by blending OEC obtained from pearl oysters genetically improved by selective breeding for traits related to pearl quality. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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