Effects of roscovitine on maintenance of the germinal vesicle in horse oocytes, subsequent nuclear maturation, and cleavage rates after intracytoplasmic sperm injection

Autor:	Katrin Hinrichs, G. E. Seidel, L. C. Franz, Edward L. Squires, Young-Ho Choi
Rok vydání:	2003
Předmět:	Genetics Embryology Germinal vesicle medicine.medical_treatment Obstetrics and Gynecology Horse Embryo Cell Biology Biology Cleavage (embryo) Intracytoplasmic sperm injection Andrology Endocrinology Reproductive Medicine Meiosis Mole medicine
Zdroj:	Reproduction. :693-700
ISSN:	1741-7899 1470-1626
Popis:	This study was conducted to evaluate the effects of roscovitine on suppression of meiosis, subsequent meiotic maturation, and cleavage rates after intracytoplasmic sperm injection of horse oocytes. Oocytes were classified as having compact or expanded cumuli (Com or Exp oocytes) and were divided into three culture groups: 30 h culture in maturation medium (30 h Mat); 54 h culture in maturation medium (54 h Mat), or 24 h culture in medium containing 66 micro mol roscovitine l(-1) and then 30 h culture in maturation medium (Ros+M). After maturation, oocytes were subjected to intracytoplasmic sperm injection and cultured in G1.2 medium for 96 h. Among oocytes fixed immediately after roscovitine culture, 26 of 31 (84%) Com oocytes and 16 of 28 (57%) Exp oocytes were at the germinal vesicle stage (P or = two normal nuclei (63 versus 36%; P or = two normal nuclei. The average number of nuclei in embryos at 96 h was significantly higher (P
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::bb881cbbf14576c73f2b6e2783f32293 https://doi.org/10.1530/rep.0.1250693 Zobrazit plný text záznamu