Cloning and Sequential Analysis of the Cytokine from Mouse

Autor:	Ai Dong Qian, Chun Fang Wang, Chun Feng Wang, Hua Rui Qi, Xiu Yun Jiang, Jia Yi Qiu
Rok vydání:	2014
Předmět:	Cloning Messenger RNA Cytokine medicine.anatomical_structure Complementary DNA medicine.medical_treatment General Engineering medicine Spleen Cytokine genes Biology Molecular biology Gene
Zdroj:	Advanced Materials Research. :2340-2343
ISSN:	1662-8985
DOI:	10.4028/www.scientific.net/amr.1030-1032.2340
Popis:	To analyze the diversity of expression quantity and lay foundation for real time fluorescent quantitation PCR, spleen samples of mouse were collected and mRNA were abstracted, furthermore, the mRNA were reverse transcribed into cDNA by using kit. Six cytokine genes IL-6, IL-12a, IL-12b, IFN-γ, TNF-αand iNOS were cloned and sequenced. The results demonstrated that the homogeneities of the sequences between amplified genes and cytokine genes in genebank all reached above 99%.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::bb7c07b5c905a81b41795d158c4a027d https://doi.org/10.4028/www.scientific.net/amr.1030-1032.2340 Zobrazit plný text záznamu