THE EFFECT OF DIET ENRICHED WITH PYROPHOSPHATE (E450) ON EXPRESSION OF GENES ENCODING BONE MORPHOGENETIC PROTEIN AND OSTEOCALCIN IN MOUSE EMBRYONIC MANDIBLE TISSUES
| Autor: | Inessa I Yakubova, Volodymyr I Ostrianko, Lesya V. Tumanovska, Victor E. Dosenko |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty biology Rodent business.industry Embryo 030206 dentistry General Medicine Bone morphogenetic protein Bone morphogenetic protein 2 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Endocrinology Internal medicine biology.animal Gene expression medicine Osteocalcin biology.protein Gestation business Gene |
| Zdroj: | Wiadomości Lekarskie. 74:43-47 |
| ISSN: | 0043-5147 |
| DOI: | 10.36740/wlek202101108 |
| Popis: | Objective The aim: Of our study was to measure the mRNA expression of the investigated odontogenesis factors in mandible tissue of mouse embryos (17th day of pregnancy) gestated by females, kept on a E450 rich diet since 30 days before fertilization to gestation. Patients and methods Materials and methods: The effect of food supplements was studied in «Overload phosphates model». Experiments were carried out on white nonlinear outbred mice with mass 25-28g (n=40). The females from the control group were fed with standard rodent food, whereas the experimental females were fed with pyrophosphate-enriched food. The materials, used for the molecular genetic study, were the lower jaws of 17-days old mouse embryos (E-17). Results Results: The investigated BMP2 and osteocalcin genes are expressed at approximately the same level. Pyrophosphate-rich diet does not alter BMP2 gene expression, but it significantly increases the expression of osteocalcin. Conclusion Conclusions: The present study is the first one to describe the impact of the pyrophosphate-rich diet on mRNA expression of key osteogenesis regulators - osteocalcin and BMP2. |
| Databáze: | OpenAIRE |
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