AChE localization in adhesive papillae of ascidian larva: effects of citral, a retinoic acid synthesis inhibitor
| Autor: | Silvia Groppelli, Robert A Pennati, Fiorenza De Bernardi, Cristina Sotgia |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Larva
integumentary system biology urogenital system Aché Retinoic acid Retinol biology.organism_classification Citral Acetylcholinesterase language.human_language Staining Cell biology chemistry.chemical_compound stomatognathic system chemistry Biochemistry language Animal Science and Zoology Ciona intestinalis Developmental Biology |
| Zdroj: | Invertebrate Reproduction & Development. 40:95-102 |
| ISSN: | 2157-0272 0792-4259 |
| Popis: | Summary Acetylcholinesterase (AChE) activity has been localized in the primary neurons of the adhesive papillae of Ciona intestinalis and Phallusia mammillata larvae using the histochemical staining method described by Karnovsky and Roots (1964) after short fixation. The shape and cell structure of the papillae and the arrangement of primary neurons are different in the two species. Anterior truncation caused by RA or retinol suppresses the differentiation of the papillae and of papillary primary neurons, but combined treatment with retinol and citral can rescue the formation of rather normal papillae with AChE positive primary neurons. The AChE staining method has proved to be a useful tool in revealing differences in the cellular structure of adhesive papillae of the two species and to investigate the differentiation of anterior ectodermal structures. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|