Phytochemicals and silk proteins in mature silkworm powders responsible for extended life expectancy and enhanced resistances to Parkinson's disease

Autor: Young Han Park, Kee-Young Kim, Sang-Deok Ji, Bo-Hye Choi, Jong Gon Son, Phuong Nguyen, Young Ho Koh
Rok vydání: 2017
Předmět:
Zdroj: Journal of Asia-Pacific Entomology. 20:1425-1433
ISSN: 1226-8615
DOI: 10.1016/j.aspen.2017.10.011
Popis: Recently, we have reported that steamed and freeze-dried mature silkworm powder (SMSP) exerts biopharmaceutical effects, such as extending the healthy life span and increasing resistance to the onset of Parkinson's disease (PD) in animal models. Freeze-dried mulberry leaves (FDML), freeze-dried 3rd day fifth instar silkworm powder (FDSP), freeze-dried mature silkworm powder (FMSP), and SMSP were extracted with 4 different solvents and used in antioxidant activity assays, cell proliferation assays, and Drosophila behavioral assays to identify the components of SMSP responsible for the extension of the life span and enhanced resistance to rotenone-induced PD. Compared with those in the FDML, the phytochemical concentrations and antioxidant activities were significantly reduced in the three silkworm powders (SPs). However, the three SPs exhibited significantly greater inhibition of lipid peroxidation than the FDML. In addition, concentrates of 80% methanol (MeOH) extracts of SMSP (CMeSP) did not display any or only very low cytotoxicity, whereas concentrates from the other samples showed significant cytotoxicity, even at very low concentrations. Furthermore, the resistance to PD observed in SMSP food (SMSPf)-raised flies was accomplished by raising flies with foods containing either CMeSP or the dried portion remaining after the 80% MeOH extraction of SMSP (DMeSP), whereas the extended life expectancy of SMSPf-raised flies was only recapitulated when CMeSP and DMeSP were provided simultaneously. Substances inhibiting the onset of rotenone-induced PD were likely phytochemicals in CMeSP or MS-derived digestible silk proteins in DMeSP. In contrast, the extension of life spans in normal individuals required substances found in both CMeSP and DMeSP, suggesting that the anti-aging mechanisms are more necessary for regulating the complex signaling pathways than the inhibition of the onset of PD.
Databáze: OpenAIRE
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