Improved method for the preparation of nonyl acridine orange analogues and utilization in detection of cardiolipin
| Autor: | Pavels Dimitrijevs, Pavel Arsenyan, Ilona Domracheva |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0303 health sciences
Trimethylsilyl Silylation Acridine orange General Chemistry Buffer solution 010403 inorganic & nuclear chemistry 01 natural sciences Fluorescence Catalysis 0104 chemical sciences 03 medical and health sciences chemistry.chemical_compound chemistry Materials Chemistry Cardiolipin Moiety Solubility 030304 developmental biology Nuclear chemistry |
| Zdroj: | New Journal of Chemistry. 44:9626-9633 |
| ISSN: | 1369-9261 1144-0546 |
| Popis: | This study was designed to develop a fast and convenient methodology for the preparation of 10-nonyl acridine orange (NAO) and its silyl analogues to improve their photo-physical properties for the detection and quantification of cardiolipin (CL). Optimized conditions allow the effective synthesis of NAO analogues with good yield and excellent purity. The introduction of a 3-(trimethylsilyl)propyl moiety improves the dye's solubility and stability in buffer solution and increases its emission intensity by ≈30%. The novel dye can be used for the selective quantification of CL in a liposomal inner mitochondrial membrane model with greater fluorescence intensity and linear slope compared to NAO. The novel silicon-containing NAO analogue has lower cytotoxicity, and is a convenient fluorescent dye for cell staining. |
| Databáze: | OpenAIRE |
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