Estrogenic and safety evaluation of root extract of Millettia aboensis as a potential plant derived alternative for hormone replacement therapy
| Autor: | Amara Anwuchaepe Ajaghaku, Daniel Lotanna Ajaghaku, Festus B. C. Okoye, FA Onyegbule |
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| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
Chemistry medicine.medical_treatment Estradiol valerate Genistein Hormone replacement therapy (menopause) Plant Science Pharmacology medicine.disease 01 natural sciences Effective dose (pharmacology) Acute toxicity 0104 chemical sciences Menopause 010404 medicinal & biomolecular chemistry chemistry.chemical_compound medicine Phytoestrogens Daidzin 010606 plant biology & botany medicine.drug |
| Zdroj: | South African Journal of Botany. 140:123-134 |
| ISSN: | 0254-6299 |
| DOI: | 10.1016/j.sajb.2021.03.040 |
| Popis: | Poor compliance with conventional estrogen replacement therapy due to associated side effects has led to the need for alternative therapeutic intervention to mitigate the symptoms and diseases associated with menopause. This study investigated the estrogenic effect of root extract of M. aboensis (MA) as a potential plant derived alternative for hormone replacement therapy (HRT). Phytoestrogen content of the methanol root extract and its solvent fractions was determined using genistein as standard. Further characterization of the phytoestrogen rich fraction (PRF) was done using HPLC-DAD. Acute toxicity of the extract was determined across oral doses of 100–5000 mg/kg while its effective dose (ED50) was tested at doses ranging from 100–1600 mg/kg using vaginal cytology as an index of estrogenicity. Uterotrophic effect, serum estradiol quantification, liver and kidney toxicities were tested following 30 days treatment of ovariectomized female rats with PRF. The LD50 of the extract was estimated to be >5000 mg/kg while the ED50 was calculated to be 321.58 mg/kg. The ethyl acetate fraction showed the highest phytoestrogen content of 115 mg genesteinEq/g. HPLC-DAD analysis of this fraction identified these phytoestrogens as 9-alpha-OH-pinoresinol, isoprunetin 8C glucoside, daidzin, genistein 6C glucoside and Neobavaisoflavone. At 200 mg/kg, PRF showed serum estradiol level of 3.16 pg/mL against 0.25 and 5.82 pg/mL shown by the vehicle and estradiol control groups respectively. Administration of PRF showed a dose dependent weekly increase in vaginal cornification. Treatment with 1 mg/kg estradiol valerate and 400 mg/kg PRF significantly (p |
| Databáze: | OpenAIRE |
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