Beta-Defensin 126 on the Surface of Macaque Sperm Mediates Attachment of Sperm to Oviductal Epithelia1
| Autor: | Ashley I. Yudin, Gary N. Cherr, Alice F. Tarantal, James W. Overstreet, Theodore L. Tollner, Cathy A. Treece |
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| Rok vydání: | 2008 |
| Předmět: |
endocrine system
biology urogenital system Cell Biology General Medicine Sperm Sialic acid Andrology chemistry.chemical_compound Beta defensin Reproductive Medicine chemistry Capacitation Immunology biology.protein Oviduct Cyclic adenosine monophosphate Caffeine Neuraminidase reproductive and urinary physiology |
| Zdroj: | Biology of Reproduction. 78:400-412 |
| ISSN: | 1529-7268 0006-3363 |
| Popis: | Beta-defensin 126 (DEFB126) coats the entire surface of macaque sperm until sperm become capacitated, and the removal of DEFB126 from over the head of sperm is required for sperm-zona recognition. Viable sperm collected from cervix and the uterine lumen of mated female macaques had DEFB126 coating the entire surface, suggesting that DEFB126 is retained on sperm en route to the oviduct. DEFB126 plays a major role in attachment of sperm to oviductal epithelial cells (OECs). Following treatment to either remove or alter DEFB126, sperm were coincubated with explants of OECs, which were assessed for sperm binding following rinsing to remove superficially attached sperm. Sperm treated with either 1 mM caffeine + 1 mM dibutyryl cyclic adenosine monophosphate (dbcAMP) (induces capacitation and complete release of DEFB126 from sperm), 2 mM caffeine (removes DEFB126 from over the head and midpiece but does not induce capacitation), anti-DEFB126 immunoglobulin, or neuraminidase (cleaves sialic acid from ter... |
| Databáze: | OpenAIRE |
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