| Popis: |
Background: Long noncoding RNAs (lncRNAs) were found to play important roles in the pathogenesis of FGR. Herein, we aimed to elucidate the role of LINC00881 in regulating various biological events of FGR.Methods: LINC00881 expression level was examined in the normal pregnancy placentas and FGR patients by quantitative reverse transcription PCR (qRT-PCR). The assays of CCK8, transwell and flow cytometry were performed to evaluate the functional effects of overexpression and silencing of LINC00881 on the HTR8/SVneo trophoblast cell line. RNA Pull down and immunoprecipitation (RIP) assays were futher used to verify the putative relationship between LINC00881 and its potential target of FSCN1.Findings: LINC00881 expression was signifcantly downregulated in 20 FGR placental tissues compared to 20 healthy placental tissues. LINC00881 overexpression promotes proliferation, migration, invasion and apoptosis of HTR8/SVneo trophoblast cell line in vitro. Downregulation of LINC00881 leads to the opposite results. RNA pull‑down assay and RIP assays reveals FSCN1 as a possible target of LINC00881. Futhermore, FSCN1 overexpression eliminated the effect of LINC00881 overexpression in HTR-8 cells. |
