| Autor: |
Papp Edina, Vancsik Tamas, Meggyeshazi Nora, Kovago Csaba, Andocs Gabor, Szasz Oliver, Krenács Tibor |
| Rok vydání: |
2015 |
| Předmět: |
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| Zdroj: |
European Journal of Cancer. 51:S7 |
| ISSN: |
0959-8049 |
| DOI: |
10.1016/j.ejca.2015.01.031 |
| Popis: |
Background Some hyperthermia studies in oncology deals with connected immune-effects. Modulated electro-hyperthermia (oncothermia, Szasz et al. (2010) [1] ) is an emerging curative treatment method. It is selectively focused in the malignant cells without harming the non-malignant surrounding tissues Andocs et al. (2009) [2] . Aim Our objective is studying the cell-death process caused by the treatment. Method HT29 human colorectal xenograft and C26 mouse colorectal allograft models were studied in vivo, on in time-course investigations. We apply 13.56 MHz radiofrequency (RF) signal modulated by 1/f noise pattern. In silico models were performed visualising the selection process. Samples were analysed by various histomorphological and immunhistochemical analyses. Results A massive TUNEL positivity was produced. Up regulation of TRAILR2 (DR5), FAS and FADD was observed. AIF nuclear translocalisation together with mitochondrial pore formation and cytochrome-C release, as well as DNA fragmentation was measured. A damage associated molecular pattern (DAMP) was formed, which are probably parts of the immunogenic cell-death (ICD) of the selected malignant cells. Conclusion The DAMP associated, induced ICD can be a good basis for hyperthermia and immunotherapy combination. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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