Effects of human serum on the stability and cytotoxicity of silver nanoparticles

Autor: A. Jezierski, Lu Yang, V. Gies, D. C. Kennedy
Rok vydání: 2019
Předmět:
Zdroj: SN Applied Sciences. 1
ISSN: 2523-3971
2523-3963
DOI: 10.1007/s42452-019-1480-6
Popis: Silver nanoparticles are ubiquitous in today’s commercial marketplace. Despite decades of research, there is still disagreement about the risk that silver nanoparticles pose to human and environmental health. There is often a lack of correlation between measured physical properties and the measured biological endpoints, making it difficult to establish trends, and discrepancies between different cell models have not been clearly accounted for. Here we have used human AB serum in cell culture media for the investigation in place of fetal bovine serum (FBS) which is typically used. In HepG2 cells, particle uptake was clearly correlated with smaller sizes of particles and with increased cytotoxicity. In neuron-like SH-SY5Y cells, similar trends were not observed. Compared with previously published data on the same particles in these cell lines with cell culture media supplemented with FBS, there are clear and significant differences in the stability of the particles, their cellular uptake and their cytotoxicity in these two commonly used cell models for in vitro cytotoxicity testing. This highlights the need for improved in vitro testing for nanomaterials and the importance of using human serum to better predict human in vivo outcomes.
Databáze: OpenAIRE