High-Level Production of Indole-3-acetic Acid in the Metabolically Engineered Escherichia coli
| Autor: | Jianzhong Huang, Yang Jinhua, Wu Hongxuan, Feng Qi, Xianzhang Jiang, Lina Qin, Weibin Wu, Li Qingchen, Xiao Cao, Shen Peijie |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
Tryptamine biology 010401 analytical chemistry food and beverages Heterologous General Chemistry Metabolism biology.organism_classification medicine.disease_cause 01 natural sciences 0104 chemical sciences chemistry.chemical_compound chemistry Biochemistry Biosynthesis medicine heterocyclic compounds NAD+ kinase Plant hormone General Agricultural and Biological Sciences Indole-3-acetic acid Escherichia coli 010606 plant biology & botany |
| Zdroj: | Journal of Agricultural and Food Chemistry. 69:1916-1924 |
| ISSN: | 1520-5118 0021-8561 |
| DOI: | 10.1021/acs.jafc.0c08141 |
| Popis: | Indole-3-acetic acid (IAA) is a critical plant hormone that regulates cell division, development, and metabolism. IAA synthesis in plants and plant-associated microorganisms cannot fulfill the requirement for large-scale agricultural production. Here, two novel IAA biosynthesis pathways, tryptamine (TAM) and indole-3-acetamide (IAM), were developed for IAA production by whole-cell catalysis and de novo biosynthesis in an engineered Escherichia coli MG1655. When 10 g/L l-tryptophan was used as a substrate, an MIA-6 strain containing a heterologous IAM pathway had the highest IAA titer of 7.10 g/L (1.34 × 103 mg/g DCW), which was 98.4 times more than MTAI-5 containing the TAM pathway by whole-cell catalysis. De novo IAA biosynthesis was optimized by improving NAD(P)H availability, resulting in an increased IAA titer of 906 mg/L obtained by the MGΔadhE::icd strain, which is 29.7% higher than the control. These strategies exhibit the potential for IAA production in engineered E. coli and possible industrial applications. |
| Databáze: | OpenAIRE |
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