Targeted Overexpression of Galanin in Lactotrophs of Transgenic Mice Induces Hyperprolactinemia and Pituitary Hyperplasia1
| Autor: | James F. Hyde, N. Patel, J. D. Hayes, Aihua Cai |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Genetically modified mouse
endocrine system Messenger RNA Pituitary gland medicine.medical_specialty Transgene Hyperplasia Biology medicine.disease Prolactin cell Endocrinology medicine.anatomical_structure Anterior pituitary Internal medicine medicine Galanin hormones hormone substitutes and hormone antagonists |
| Zdroj: | Endocrinology. 140:4955-4964 |
| ISSN: | 1945-7170 0013-7227 |
| DOI: | 10.1210/endo.140.11.7120 |
| Popis: | We generated transgenic mice that carry 4.6 kb of the mouse galanin gene fused to 2.5 kb of the rat PRL promoter. In all transgenic lines that carried and transmitted the transgene, there were significant increases in galanin messenger RNA and peptide levels in the anterior pituitary in both male and female transgenic mice, and the elevation of galanin was restricted to the anterior lobe. Furthermore, galanin release from pituitary cells in vitro of both male and female transgenic mice was dramatically increased compared with that in control mice. At 2–4 months of age, pituitary PRL contents in female transgenic mice were increased compared with those in normal controls. Moreover, PRL messenger RNA levels were increased in female transgenic mice. However, plasma levels of PRL in female transgenic mice were not significantly higher until 6 months of age. By 11 months of age, cell numbers in the anterior pituitary were increased in female, but not male, transgenic mice. The percentage of lactotrophs in fema... |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|